Determination of Total Flavonoid and Antibacterial Activity of Parameria laevigata (Juss.) Moldenke Bark Extract Against Staphylococcus saprophycitus and Escherichia coli
Keywords:
Escherichia coli, Flavonoid Total, Rapet Bark, Staphylococcus saprophycitus, UAEAbstract
Parameria laevigata (Juss.) Moldenke Bark is a natural ingredient containing metabolic secondary and empirically has been used to support health. This study aims to determine total flavonoid content of extract rapet bark using 70% and 96% ethanol by UAE (Ultrasonic-Assisted Extraction) for one hour at 40ºC, determine antibacterial activity of extraxt rapet bark containing the highest total flavonoid content, and to determine the differences in antibacterial activity of extract rapet bark containing flavonoid content at concentrations 1, 3, 5% against Staphylococcus saprophycitus and Escherichia coli. The extraction process was carried out by UAE with a ratio 1:10. Extract results were subjected to ethanol free, color reaction test, TLC, determination of total flavonoid content, and the results of the highest total flavonoid content will be tested for antibacterial activity. The obtained ethanol content of 70% was 19.8737 ± 0.5828 mgRE/gram, 12.8389 ± 0.1687 mgRE/gram in ethanol 96%, and sequentially obtained antibacterial inhibition zones of 8.750 ± 0.025 mm; 9.855 ± 0.021; 12.460 ± 0.038 mm in Staphylococcus saprophycitus and 13.635 ± 0.029 mm; 14.545 ± 0.033 mm; 15.455 ± 0.021 in Escherichia coli.
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