Inhibition of Staphylococcus aureus Biofilm by Ethanol Extract of Nasturtium officinale
Keywords:
Biofilm Assay, Extract, Microtiter Plate, Watercress LeavesAbstract
The Staphylococcus aureus biofilm formation can increase antibiotic resistance in chronic diseases including wound infections. In a biofilm state, susceptibility to antibiotics is reduced, making it more difficult to eradicate bacteria. Watercress (Nasturtium officinale) is known to contain compounds that have the potential to be antibacterial and antibiotic agents. This study aims to determine the inhibition activity of the formation and degradation of Staphylococcus aureus biofilm from watercress leaf ethanol extract with concentrations of 1%, 5%, and 25%, as well as to determine the content of antibiofilm compounds in the extract. Nasturtium officinale ethanol extract is obtained by remaceration. Antibiofilm testing was performed with Microtiter Plate Biofilm Assay, and ciprofloxacin was used as a positive control. The results of the one-way anava test showed differences in activity both in inhibition of formation and degradation of Staphylococcus aureus biofilm. The extract with a concentration of 25% provided the greatest activity, which was 63.00% inhibition of biofilm formation and 52.77% biofilm degradation. Flavonoid compounds, phenolics, tannins, saponins, and steroids in the extract have the potential to provide activity.
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