Antiinflammatory Activity Test Through Method Denaturation of Bovine Serum Albumin (BSA) Protein Extract and Fraction of Eucheuma cottonii
Keywords:
Anti-inflammatory, BSA, Eucheuma cottonii, Extract, FractionAbstract
Inflammation is the body's protective response to harmful stimuli, but chronic inflammation can trigger various degenerative diseases, so safer anti-inflammatory alternatives from natural ingredients are needed. This study aims to evaluate the anti-inflammatory activity of Eucheuma cottonii from several regions using the Bovine Serum Albumin (BSA) protein denaturation inhibition method and to examine the effect of fraction polarity on the resulting activity. Samples were extracted by the remaceration method using 70% ethanol, then the most active extract was fractionated through liquid-liquid partitioning using n-hexane, ethyl acetate, and water. Anti-inflammatory activity was tested in vitro based on the percentage of inhibition and IC50 values measured with a UV-Vis spectrophotometer at a wavelength of 660 nm, using diclofenac sodium as a positive control. The results showed that extract C had the highest activity with an inhibition percentage of 79.92% ± 0.26%, while the ethyl acetate fraction of the most active extract showed the lowest IC₅₀ value (146.53 µg/mL ± 4.10%) compared to the n-hexane and water fractions. Statistical analysis showed a significant difference in anti-inflammatory activity between extracts and fractions (p < 0.05), which indicated that the sample origin and the polarity level of the fractions affected the anti-inflammatory activity of Eucheuma cottonii.
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